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Characteristics of Liposomes Prepared by the Modified Reverse‐Phase Evaporation Method: 31 P‐NMR and TEM Study
Author(s) -
Pentak Danuta,
Sułkowski Wiesław W.,
Maślanka Sławomir,
Wolińska Agnieszka,
Duber Stanisław,
Sułkowska Anna
Publication year - 2006
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200651368
Subject(s) - liposome , materials science , vesicle , transmission electron microscopy , evaporation , electron micrographs , nuclear magnetic resonance spectroscopy , phase (matter) , analytical chemistry (journal) , chromatography , chemistry , nanotechnology , electron microscope , membrane , organic chemistry , optics , thermodynamics , biochemistry , physics
The NMR spectroscopy was used to determine a structure and physicochemical properties of liposomes prepared from L ‐α‐phosphatidylcholine dipalmitoyl (DPPC) by the modified reverse‐phase evaporation method (mREV). 31 P‐NMR study of the liposome in the temperature range from 340K to 350K allowed us to suggest that the single‐bilayered liposome has been obtained. Transmission electron micrographs (TEM) showed that the liposome vesicles, obtained with the use of the modified (mREV) method, were spherical in shape and in majority they were less than 100 nm in diameter.