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Efficient DNA Extraction and Amplification of Samples from the Archeological Site of Pompei (Italy)
Author(s) -
Ranalli Giancarlo,
Belli Claudia,
Lustrato Giuseppe,
Meloscia Antonia,
Orsini Massimiliano
Publication year - 2006
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200650614
Subject(s) - dna extraction , extraction (chemistry) , dna , cultural heritage , polymerase chain reaction , ancient dna , reagent , biology , archaeology , microbiology and biotechnology , chemistry , geography , genetics , chromatography , gene , population , demography , sociology
Due to their growth and metabolism, microorganisms can cause severe damage to Cultural Heritage, through mechanical and chemical processes. In this paper we used molecular approaches to study bacterial communities on samples from the archeological site of Pompei (Italy). In particular four DNA extraction methods without prior cultivation of the microflora were compared. In terms of efficiency, the DNA extraction method based on the Tri‐Reagent™ kit gave the best results. All extraction protocols coupled with 16S rDNA fragments PCR amplification were satisfactory; but the best results were obtained by Klentaq DNA LA Polymerase™; this enzyme can be considered an effective tool for amplification of DNA from Cultural Heritage and possibly other environmental samples.