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Influence of Heat Treatment on Biological Properties of Chitosan toward Vascular Cells in vitro
Author(s) -
Lim SaeHwan,
Park KiSook,
Park HyunSook,
Gin YongJae,
Noh InSup,
Park ChanWoong,
Son YoungSook,
Kim ChunHo
Publication year - 2005
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200550623
Subject(s) - chitosan , in vitro , chemistry , cell growth , materials science , biophysics , biochemistry , biology
Abstract The effects of heat treatment of chitosan film on the proliferation of vascular cells were examined in vitro . Chitosan film was heat treated at 110 °C in vacuo for 1∼3 days. Heat treatment slightly improved the hydrophilicity of chitosan, although the cross‐linking of chitosan took place after heat treatment. Chitosan films were cytocompatible toward smooth muscle cells (SMCs) and human dermal microvascular endothelial cells (HDMECs) before and after heat treatment of chitosan. In particular, the heat treated chitosan supported the proliferation of both vascular cell types for one day. The growth of HDMECs was greater than that of SMCs on heat treated chitosan film for one day. Heat treated chitosan has intrinsic biological activity which could contribute to a retardation of SMC growth relative to HDMECs.