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Studying Spatial Distributions of Influenza Hemagglutinin on the Plasma Membrane of Fibroblasts: A Work in Progress
Author(s) -
Zimmerberg Joshua,
Kumar Mukesh,
Verma Anil,
Farrington Jane,
Roth Michael,
Kenworthy Anne,
Hess Samuel T.
Publication year - 2004
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200550103
Subject(s) - hemagglutinin (influenza) , immunogold labelling , förster resonance energy transfer , membrane , cell fusion , microbiology and biotechnology , viral envelope , fluorescence microscope , biophysics , virus , raft , lipid bilayer fusion , biology , lipid raft , cell , chemistry , virology , fluorescence , ultrastructure , biochemistry , anatomy , physics , polymerization , organic chemistry , polymer , quantum mechanics
The major envelope protein of influenza virus, hemagglutinin (HA), mediates the fusion of virus to cell for infection, and can mediate cell‐cell fusion. It has been studied as a “raft” protein, as it is found in detergent‐resistant membranes (DRM) and trafficks apically in polarized epithelia. Moreover, the viral envelope of influenza itself is rich in sphingomyelin and cholesterol. Using both immunogold electron microscopy and fluorescence resonance energy transfer (FRET) microscopy, we are examining the distribution of HA on the surface of fibroblasts expressing wild‐type HA.