Premium
Complexes of cellulose and trypsin
Author(s) -
Kotelnikova Nina E.,
Mikhailova Sofia A.,
Vlasova Ele.
Publication year - 2004
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.200450649
Subject(s) - microcrystalline cellulose , trypsin , cellulose , microcrystalline , adsorption , chemistry , desorption , aqueous solution , chemical engineering , materials science , chromatography , organic chemistry , crystallography , enzyme , engineering
Adsorption of trypsin to microcrystalline cellulose has been determined as functions of protein concentration and pH of the aqueous medium. The study of adsorption at several pH values indicates that interaction of trypsin to the microcrystalline cellulose interface is controlled by the electrostatic effect. The FTIR, desorption, and SEM data reveal that a part of trypsin is strongly bound to the microcrystalline cellulose matrix. Resulting complexes consist of microcrystalline cellulose, trypsin, and water.