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Deposition and bioadhesion properties of polymer multilayers: An in‐situ ‐ATR‐FTIR‐study
Author(s) -
Müller Martin,
Rieser Theresia,
Köthe Marcello,
Keßler Bernd,
Brissova Marcela,
Lunkwitz Klaus
Publication year - 1999
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.19991450116
Subject(s) - polyelectrolyte , attenuated total reflection , acrylic acid , fourier transform infrared spectroscopy , adsorption , chemical engineering , polymer , chemistry , polymer chemistry , infrared spectroscopy , layer by layer , allylamine , maleic acid , layer (electronics) , materials science , copolymer , organic chemistry , engineering
The multilayer formation by consecutive deposition of oppositely charged polyelectrolytes and the protein adsorption at the outermost layer was investigated by in‐situ attenuated total reflection Fourier transform infrared (ATR‐FTIR) spectroscopy. For the polyelectrolyte multilayer generation the polycation / polyanion pairs poly(diallyldimethylammonium chloride) (PDADMAC) / sodium poly(maleic acid‐co‐propylene) (PMAPP) and poly(ethyleneimine) (PEI) / poly(acrylic acid) (PAC) respectively, were used. The v(CO)‐ and the v as (COO)‐band due to COOH‐ und CO − ‐groups of the polyacid/polyanion as well as the v(OH) band due to desorbed water were used for monitoring the multilayer build up. By the multilayer surface modification we were able to diminuish or enhance significantly the adsorption of the model plasma protein human serum albumine (HSA), as a measure for bioadhesion, in dependence on the character of the outermost layer. This protein resistence mainly was attributed to the repulsion between the like charges of the outermost adsorbed polyanion layer (e.g. PAC) und the acidic HSA (IEP=4.7). Vice versa it could be shown, that HSA was bound by an outermost polycation layer by a factor >10 stronger compared to an outermost polyanion layer. Furthermore studies on protein adsorption at multilayers built up by the reactive polymer pair poly(butadienepoxide) (PBDE) / poly(allylamine) (PAA) were introduced.