Premium
Electrochemical polymerization of functionalized thiophene derivatives for the immobilization of proteins
Author(s) -
Welzel HansPeter,
Kossmehl Gerhard,
Engelmann Gunnar,
Neumann Barbara,
Wollenberger Ulla,
Scheller Frieder,
Plieth Waldfried
Publication year - 1998
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.19981260123
Subject(s) - thiophene , polymer chemistry , bromide , chemistry , polymerization , copolymer , ether , organic chemistry , polymer
The hydroxy group of 3‐(2‐hydroxyethyl)thiophene was protected as methyl ether 1 and as dimethyl tert‐butyl silyl ether 5 before anodic polymerization. The poly[3‐(2‐methoxyethyl)thiophene] 2 was prepared by electrochemical homopolymerization of 1 . Ether cleavage was carried out in the polymer film 2 and the resulting poly [3‐(2‐hydroxyethyl)thiophene] ( 3 ) was activated with cyanogen bromide to immobilize alcohol dehydrogenase. Silylether 5 did not undergo homopolymerization but copolymerization of 5 with 3‐methylthiophene ( 4 ) was successful. After cleavage of the protecting group the resulting copolymer 7 was activated by cyanuric chloride, and chymotrypsin was immobilized. Electrocopolymerization of thiophene‐3‐acetic acid ( 8 ) and 3‐methylthiophene ( 4 ) under various conditions produces copolymer 9 . By activation of the carboxylic groups with N,N'‐dicyclohexylcarbodiimide (DCC) lactate oxidase (LOD) was bond to the surface of the electrode to form a lactate sensor.