Premium
High performance liquid chromatography characterization of macromolecules
Author(s) -
Chang Taihyun,
Lee Hee Cheong,
Lee Wonmok
Publication year - 1997
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.19971180136
Subject(s) - macromolecule , size exclusion chromatography , polymer , chromatography , characterization (materials science) , alkyl , materials science , elution , resolution (logic) , hydrophilic interaction chromatography , chemistry , high performance liquid chromatography , organic chemistry , nanotechnology , composite material , biochemistry , artificial intelligence , computer science , enzyme
A new method, temperature gradient interaction chromatography(TGIC) is employed for the characterization of macromolecules. Fine and reproducible control of interaction between polymer chains and the alkyl chain bonded silica packing material can be achieved by varying the temperature of the column. This method provides a far superior resolution to the conventional size exclusion chromatography. In addition, this method has a high sample loading capacity to be effective for preparative purpose. Furthermore, this method can be used to characterize binary polymer mixtures, where one component of a polymer mixture is separated by the size exclusion mechanism and the other is by the interaction mechanism simultaneously from single isocratic elution.