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Polymer ultrathin films with immobilized photosynthetic reaction center proteins
Author(s) -
Kalabi. A.,
Zaitsev S. Yu.,
Zubov V. P.,
Lukashev E. P.,
Konko A. A.
Publication year - 1996
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.19961060118
Subject(s) - monolayer , monomer , photosynthetic reaction centre , phosphatidylcholine , polymer , polymerization , phosphatidylethanolamine , chemistry , materials science , polymer chemistry , chemical engineering , organic chemistry , phospholipid , photosynthesis , nanotechnology , biochemistry , membrane , engineering
Properties of mixed monolayers of lipid‐photosynthetic reaction center proteins (RC) were studied and the optimum conditions for stable films fabrication were determined. The following synthetic: N‐acryloylphosphatidylethanolamine (ACPE), tetracosa‐11, 13‐diinoic acid (TDA), pentacosa‐10, 12‐diinoic acid (PDA), dioctadecyldienoylphosphatidylcholine (DODL) and natural lipids: L‐α‐phosphatidylethanolamine (PE), L‐α‐phosphatidylcholine (PC) were used. The rate of polymerization of the mixed ACPE‐RC and TDA‐RC monolayers is lower in comparison with corresponding values for pure lipid‐like monomers on air/water interface. The optical and photoelectrical measurements provide evidence for an orientation of RCs on interface. Hydrophilic H‐subunit in monomeric and polymeric ACPE‐RCs, and monomeric DODL‐RCs monolayers is preferentially oriented towards water as in the pure RC monolayers. Opposite orientation was found with TDA‐RCs and PDA‐RCs films. No preferential orientation for lipid‐RCs from C. aurantiacus monolayers was found because of the RCs having low assymmetry of hydrophobic subunits ( M and L ).