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Macromolecules in ordered media. II. A fluorescence study of the polymer‐liposome association
Author(s) -
Porcar Iolanda,
Gómez Clara M.,
Codoñer Armando,
Soria Vicente,
Campos Agustín
Publication year - 1995
Publication title -
macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 1022-1360
DOI - 10.1002/masy.19950940115
Subject(s) - liposome , polymer , vesicle , macromolecule , fluorescence , membrane , aqueous solution , materials science , chemical engineering , chemistry , analytical chemistry (journal) , polymer chemistry , chromatography , organic chemistry , nanotechnology , optics , biochemistry , physics , engineering
Abstract The interactions of poly (4‐vinyl pyridine) with dimyristoylphosphatidic acid‐based liposomes were studied in heterogeneous solution systems. The polymer was dispersed in aqueous buffer solutions of small unilamellar vesicles and after a suitable period of incubation to raise the equilibrium, the experiments has been conducted as a function of polymer concentration and temperature. According to the measurements of the steady‐state fluorescence spectra, changes in the intensities as well as shifts in the emission peak wavelength, has served as raw data to accounting for the polymer‐vesicle interactions. Quantitative evaluation of the interaction has been done using a simple associating model with some approximations. The key parameters evaluated were the amount of polymer bound and the number of charged lipids on a membrane site as well as the number of effective charges of poly (4‐vinyl pyridine) chains at the liposome interface.