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Monosized polymer particles in biochemical and biomedical separations
Author(s) -
Ellingsen Turid,
Aune Oddvar,
Berge Arvid,
Kilaas Lars,
Schmid Ruth,
Stenstad Per,
Ugelstad John,
Hagen Steinar,
Weng Ellen,
Johansen Liv
Publication year - 1993
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19930700133
Subject(s) - dispersity , polymer , biomolecule , particle (ecology) , chemical engineering , phase (matter) , materials science , size exclusion chromatography , chromatography , particle size , chemistry , aqueous two phase system , aqueous solution , nanotechnology , polymer chemistry , organic chemistry , oceanography , geology , engineering , enzyme
The method of activated swelling allows production of highly monodisperse polymer particles in the range of 1 to 100μm, with identical bead‐to‐bead morphology. Monosized particles with a wide variety in content of crosslinker, pore size and pore size distribution have been evaluated in different areas of biotechnology. Underivatized macroporous particles have been developed for reversed phase and size exclusion chromatography in organic solvents. Supports for aqueous phase ion exchange have been produced by covalent coupling of a hydrophilic layer to the particle surface. These media give excellent characteristics with regard to selectivity, capacity and recoveries of biomolecules. In this paper proteins and peptides are used to illustrate chromatographic performance. Monosized polymer particles have been magnetized by a process where magnetic iron oxides are formed inside preformed monodisperse particles. These particles are used extensively in selective isolation of cells and bacteria and in DNA technology. This paper reviews some new results in the field of cell separation.