Premium
Characterization of polysaccharide solutions by means of chromatography‐multidetection
Author(s) -
Huber Anton
Publication year - 1992
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19920610120
Subject(s) - amylopectin , size exclusion chromatography , polymer , molar mass distribution , gel permeation chromatography , characterization (materials science) , chemistry , chromatography , aqueous solution , analytical chemistry (journal) , refractometry , particle size , polysaccharide , starch , multiangle light scattering , intrinsic viscosity , light scattering , materials science , refractive index , scattering , optics , organic chemistry , physics , nanotechnology , optoelectronics , amylose , enzyme
Size exclusion chromatography (SEC) combined with double detection, utilizing a differential refractive index (DRI)‐ and a low angle laser light scattering (LALLS) ‐ detector represents a powerful experimental set‐up in the field of chromatography‐multidetection (C‐MD). By means of these techniques high‐quality information for profound polymer characterization is provided in a fast and semi‐automatical (autosampler/PC‐controlled) way. Molecular characteristics such as molecular weight distributions, molecular weight averages, tendencies for intermolecular interactions, can be obtained by these techniques. Successful handling of data from C‐MD‐techniques within reasonable time ranges requires adequate hardware and software. This is especially true if advanced data processing is applied, e.g. to yield polymer particle size/shape characteristics in terms of Staudinger/Mark/Houwink constants (K,a), distributions of sphere equivalent particle radii (R c D)‐, sphere equivalent mass density of solved polymer coils (ρ c D)‐ and intrinsic viscosity ([η]D). For aqueous solutions of polysaccharide samples (waxy maize starch hydrolyzate, hydroxyethyl‐amylopectin from waxy maize hydrolyzate, dextran) data from SEC‐DRI/LALLS and universal calibration experiments are utilized for a comprehensive physico‐chemical characterization of these polymers.