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Fluorescence microscopy studies of layer/substrate interaction during the Langmuir‐Blodgett transfer: Fractional condensation and local layer modification in lipid monolayers at the three‐phase line
Author(s) -
Spratte Karl,
Riegler Hans
Publication year - 1991
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19910460115
Subject(s) - monolayer , dipalmitoylphosphatidylcholine , langmuir–blodgett film , substrate (aquarium) , phase (matter) , chemistry , layer (electronics) , analytical chemistry (journal) , materials science , chemical engineering , crystallography , chemical physics , organic chemistry , nanotechnology , phospholipid , biochemistry , oceanography , membrane , phosphatidylcholine , geology , engineering
Transfer fluorescence microscopy reveals the substrate‐mediated fractional condensation and phase‐selective deposition of dipalmitoylphosphatidylcholine (DPPC) and dimyristoylphosphatidylethanolamine (DMPE) monolayers during the LB‐transfer. Preferentially the higher ordered liquid‐condensed (LC) state is transferred onto the substrate during the transfer of a monolayer in the LC/LE (liquid/expanded) coexistence state on the water subphase. This is manifested in the directly observable attraction of LC‐domains towards the three‐phase line and observation of a domain‐free gap as consequence of the segregation of the fluorescent probe into the floating monolayer adjacent to the three‐phase line. Fingering domain growth nucleating at the three‐phase line and the substrate‐mediated pressure deposition of probe‐free material corroborate the preference of the solid substrate for the higher condensed phase. These observations are caused by changes in the free energy of the monolayer due to the replacement of the aqueous interface by the solid substrate surface.