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Reversed micelles as matrix microreactors for chemical processing of macromolecules
Author(s) -
Alexander V.Kabanov
Publication year - 1991
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19910440126
Subject(s) - macromolecule , polyelectrolyte , micelle , chemistry , coacervate , pulmonary surfactant , matrix (chemical analysis) , reagent , yield (engineering) , chemical engineering , crystallography , materials science , chromatography , polymer , organic chemistry , aqueous solution , biochemistry , metallurgy , engineering
Being solubilized in the systems of the surfactant reversed micelles (RM), the macromolecules incorporate into the inner cavity of RM, whose size can be changed by varying the surfactant hydration degree W o , i.e. [H 2 O]/[Surfactant] molar ratio. The conjugates of macromolecules (protein‐protein, protein‐linear polyelectrolyte) are synthesized in RM of AOT (Aerosol OT, sodium bis[2‐ethylhexyl]sulfosuccinate) in octane. The yield critically depends on the hydration degree: the reaction does not proceed at low W o , but at W o exceeding threshold value (which differs for various macromolecules) the yield increases sharply and reaches 100%. Using the ultracentrifugation it was demonstrated that at W o lower than threshold the polyelectrolyte represents a compact globule compressed by the micellar matrix in the RM inner cavity. Under these conditions there is no space for the protein in the RM, containing polyelectrolyte, and therefore reaction does not proceed. At W o higher than threshold RM become large enough to entrap the conjugated macromolecules simultaneously. The possibility of regulation of the conjugate composition by variation of W o (size of micellar matrix) was demonstrated. The RM are applied as universal matrix microreactors, for modification of macromolecules with water insoluble reagents and for regulation of supramacromolecular composition of oligomeric enzymes.