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Spatial structure of biomolecules by nuclear relaxation and molecular modelling methods
Author(s) -
Rossi Claudio,
Picchi Maria Pia,
Marchettini Nadia
Publication year - 1990
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19900340105
Subject(s) - homonuclear molecule , heteronuclear molecule , residual dipolar coupling , relaxation (psychology) , biomolecule , chemistry , dipole , nuclear magnetic resonance spectroscopy , chemical physics , nuclear overhauser effect , nuclear magnetic resonance , molecule , physics , psychology , social psychology , biochemistry , organic chemistry
Nuclear magnetic resonance spectroscopy (NMR) has become the most frequently used technique for the investigation of the structure of biomacromolecules in solution. In particular, selective relaxation techniques have been used in the analysis of homonuclear dipolar connectivities (selective and biselective proton spin‐lattice relaxation experiments) and heteronuclear couplings (selective {H} C‐NOE, biselective carbon R 1 experiments). An accurate determination of selected i‐j internuclear distances has made it possible to identify the preferred solution structure. A different experimental technique based on two‐dimensional NOE spectra has made it possible to define the complex network of dipolar interactions that exists in biomacromolecules and hence the conformational properties in solution.