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Design of target‐specific polymer conjugates and complexes for biomedical application
Author(s) -
Kabanov Victor A.
Publication year - 1990
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19900330124
Subject(s) - conjugate , chemistry , micelle , macromolecule , combinatorial chemistry , covalent bond , biophysics , polyelectrolyte , polymer , biochemistry , biology , organic chemistry , aqueous solution , mathematical analysis , mathematics
The new effective method of synthesis of protein‐protein and protein‐water soluble linear polymer conjugates based on using of hydrated reversed micelle systems is described. Such conjugates are of a great potential importance in particular for biomedical applications. One example represents a new family of receptor specific screened immunotoxins (respecrins) whose toxic effect develops only with respect to the target but not to the other cells. Respecrin is a toxin covalently bound to a target‐equivalent macromolecule then complexed with an antitarget antibody. Another example relates to artificial immunogenes which are conjugates of antigens or haptenes with non‐natural linear polyelectrolytes. The general mechanism of a search and recognition of target immune cells by such conjugates in vivo was confirmed in model experiments using cell‐mimetic particles. It is also shown that complexing of the DNA plasmide with the carbochain polycation results in formation of membrane active polycomplex much more active in transformation of competent cells than pure plasmide.