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Enzymatic release of drug from polymer matrices: Diffusion and activity of lysozyme within ophtalmic hydrogels
Author(s) -
CassianiIngoni L.,
Subira F.,
Bunel C.,
Vairon JP.,
Halary JL.
Publication year - 1988
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19880190123
Subject(s) - lysozyme , self healing hydrogels , penetration (warfare) , methacrylate , chemistry , polymer , copolymer , polymer chemistry , fluorescence microscope , sulfonate , diffusion , substrate (aquarium) , enzyme , fluorescence , chemical engineering , biophysics , organic chemistry , biochemistry , sodium , physics , oceanography , operations research , quantum mechanics , biology , engineering , thermodynamics , geology
Diffusional behaviour of lysozyme into a high‐hydrated hydrogel, used as precursor of contact lenses, is reported. The gel is a swollen statistical copolymer of methyl methacrylate and N‐vinyl‐2‐pyrrolidone (≁ 70% water), and the penetration of the enzyme, labeled with 8‐anilinonaphthalene‐1‐sulfonate, was followed by fluorescence microscopy. Mathematical analysis of the profiles led to the diffusion parameters (D ≁ (2–6) · 10 −8 cm 2 · s −1 ). It is shown that the enzymatic activity persists when the lysozyme and the cleavable substrate are trapped in the matrix.