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Polymeric supports for affinity chromatography and high‐performance affinity chromatography
Author(s) -
ErnstCabrera Karin,
Wilchek Meir
Publication year - 1988
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19880190111
Subject(s) - affinity chromatography , chemistry , ligand (biochemistry) , chromatography , thermoresponsive polymers in chromatography , high performance liquid chromatography , combinatorial chemistry , polymer , matrix (chemical analysis) , hydrophilic interaction chromatography , organic chemistry , biochemistry , receptor , enzyme
Affinity chromatography is the most selective chromatographic method for the purification of biologically active materials. It is based on the biospecific interaction of the substrates with a ligand, which is chemically immobilized onto a suitable matrix (support). Different matrices provided by natural and synthetic polymers are used for the preparation of affinity supports. In this communication we describe and compare the properties of various supports based on polysaccharides, polyacrylamides and inorganic materials. In particular, we discuss the utility of different silica derivatives (especially primary hydroxyl silica) for the immobilization of ligands and high‐performance affinity chromatography.