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Crosslinked polyacrylamide‐hydrazide (paah) as matrix for the gel entrapment of cells and enzymes ‐ an overview
Author(s) -
Freeman Amihay
Publication year - 1988
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19880190109
Subject(s) - entrapment , glyoxal , polyacrylamide , hydrazide , polyacrylamide gel electrophoresis , chemistry , enzyme , matrix (chemical analysis) , immobilized enzyme , chromatography , biochemistry , polymer chemistry , organic chemistry , surgery , medicine
The development and use of a gel entrapment technique designed for the immobilization of viable, sensitive cells is described. The method is based on the controlled chemical cross‐linking of prepolymerized, linear polyacrylamide partially substituted with acylhydrazide groups, by glyoxal. The methodology, applications and advantages of this technique for the immobilization of cells and enzymes by gel entrapment are reviewed.