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Immunoaffinity separations of cells in two polymer aqueous phase systems
Author(s) -
Brooks Donald E.,
Sharp K. A.,
Stocks S. J.
Publication year - 1988
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19880170129
Subject(s) - partition (number theory) , reagent , partition coefficient , polymer , chemistry , ethylene glycol , aqueous solution , derivatization , dextran , combinatorial chemistry , antibody , chromatography , organic chemistry , high performance liquid chromatography , mathematics , biology , immunology , combinatorics
Two phase systems based on aqueous mixtures of polymers such as dextran and poly(ethylene glycol) have proven to be useful in the separation of cells via partition. By suitably modifying antibodies raised against cell surface antigens so that they partition strongly in favour of one of the phases it is possible to produce selective isolations of antigenically specified cells. Derivatization of a secondary antibody which recognizes the constant region of a class of primary antibodies provides a more generally useful immunoaffinity reagent. The theory of affinity partitioning of ideal species predicts extremely large increases in partition as the result of binding of affinity ligands. Results to date indicate effects smaller than predicted by theory, however, suggesting that the polymers and other modifying agents used to change the partition behaviour of the antibodies behave non‐ideally.