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A new kind of “thiophilic” electron‐donor‐acceptor adsorbent
Author(s) -
Porath Jerker,
Oscarsson Sven
Publication year - 1988
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19880170127
Subject(s) - adsorption , chemistry , agarose , residue (chemistry) , covalent bond , protein adsorption , organic chemistry , chromatography
Four simple agarose derivatives have been synthesized: 3‐(Z‐pyridylsulfido)‐2‐hydroxypropyl‐ 3‐(2‐pyridyloxy)‐2‐hydroxypropyl‐ 3‐(phenylsulfido)‐2‐hydroxypropyl‐, and 3‐(phenyloxy)‐2‐hydroxypropylagarose. Their affinities for serum proteins have been studied in the presence and absence of water‐structuring salt. About 50% of the proteins, among them immunoglobulins and α 2 ‐macroglobulin, but not albumin, were non‐covalently adsorbed to the PyS‐gel in buffer containing 0.5 M K 2 SO 4 . These proteins are the same as those adsorbed to the so‐called thiophilic gel, T‐gel (5), but there are some additional proteins found in the adsorbate that strongly indicate a specific influence on the protein adsorption exerted by the π‐electron system of the pyridine residue. The thio‐ether sulfur appears to be a necessary structural requirement for the characteristic adsorption behaviour. Thiophilic vs. hydrophobic ligand‐protein interaction is discussed.

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