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The design of agarose beds for high‐performance hydrophobic‐interaction chromatography and ion‐exchange chromatography which show increasing resolution with increasing flow rate
Author(s) -
Hjertén Stellan,
Kunquan Yao,
Liao Jiali
Publication year - 1988
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19880170126
Subject(s) - agarose , chromatography , volumetric flow rate , resolution (logic) , chemistry , ion chromatography , hydrophilic interaction chromatography , ion exchange , porosity , elution , ion , high performance liquid chromatography , organic chemistry , physics , quantum mechanics , artificial intelligence , computer science
Agarose beads (agarose concentration: 15%; diameter: 15–70 μm) were shrunk, crosslinked and derivatized in organic solvents. As crosslinker and coupling agent γ‐glycidoxypropyl tri‐methoxysilane was used. Columns packed with nonporous beads of pentyl agarose and octyl agarose, prepared by this technique, were used for hydrophobic‐interaction chromatography. Characteristic of these columns was that the resolution increased with an increase in flow rate (except for very low flow rates). This very attractive behavior, which violates the generally accepted theory of chromatography, was also exhibited by an ion‐exchanger based on non‐porous agarose beads.