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Current development of analytical affinity chromatography: Design and biotechnological uses of molecular recognition surfaces
Author(s) -
Chaiken Irwin M.,
Shai Yechiel,
Fassina Giorgio,
Caliceti Paolo
Publication year - 1988
Publication title -
makromolekulare chemie. macromolecular symposia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.257
H-Index - 76
eISSN - 1521-3900
pISSN - 0258-0322
DOI - 10.1002/masy.19880170119
Subject(s) - affinity chromatography , molecular recognition , chemistry , peptide , chromatography , computational biology , combinatorial chemistry , enzyme , molecule , biochemistry , biology , organic chemistry
Analytical high performance liquid affinity chromatography (analytical HPLAC) has been investigated as an experimental guide to both synthetic design and affinity technological use of peptide and protein recognition surfaces. This work has progressed from the ongoing use of analytical affinity chromatography to study interaction mechanisms of naturally‐occurring peptides and proteins, including enzyme fragment complexes and neuroendocrine biosynthetic precursors. We recently initiated a study to use analytical HPLAC for de novo design of recognition peptides called “anti‐sense peptides”. Present data suggest the potential to use anti‐sense peptides as “synthetic antibodies”, in immobilized forms, for biomolecular separation and analysis. Analogous studies have been started with immobilized natural antibodies in analytical immuno HPLAC. Our present data typify the growing usefulness of analytical HPLAC when designing recognition molecules, analyzing their interaction characteristics, and devising ways to use them in affinity technology.