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BIOANALYSIS AND BIOTRANSFORMATION OF OLIGONUCLEOTIDE THERAPEUTICS BY LIQUID CHROMATOGRAPHY‐MASS SPECTROMETRY
Author(s) -
Sutton James Michael,
Kim Jaeah,
El Zahar Noha M.,
Bartlett Michael G.
Publication year - 2020
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.21641
Subject(s) - bioanalysis , chemistry , oligonucleotide , chromatography , biotransformation , mass spectrometry , liquid chromatography–mass spectrometry , biochemical engineering , dna , organic chemistry , biochemistry , engineering , enzyme
Since 2016, eight new oligonucleotide therapies have been approved which has led to increased interest in oligonucleotide analysis. There is a particular need for powerful bioanalytical tools to study the metabolism and biotransformation of these molecules. This review provides the background on the biological basis of these molecules as currently used in therapies. The article also reviews the current state of analytical methodology including state of the art sample preparation techniques, liquid chromatography‐mass spectrometry methods, and the current limits of detection/quantitation. Finally, the article summarizes the challenges in oligonucleotide bioanalysis and provides future perspectives for this emerging field. © 2020 John Wiley & Sons Ltd.