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THE PRESENT AND FUTURE OF THE MASS SPECTROMETRY‐BASED INVESTIGATION OF THE EXOSOME LANDSCAPE
Author(s) -
Xu Lian,
Gimple Ryan C.,
Lau Wayne Bond,
Lau Bonnie,
Fei Fan,
Shen Qiuhong,
Liao Xiaolin,
Li Yichen,
Wang Wei,
He Ying,
Feng Min,
Bu Hong,
Wang Wei,
Zhou Shengtao
Publication year - 2020
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.21635
Subject(s) - proteomics , chemistry , extracellular vesicles , exosome , computational biology , microvesicles , nanotechnology , data science , biology , microbiology and biotechnology , computer science , biochemistry , microrna , materials science , gene
Exosomes are critical intercellular messengers released upon the fusion of multivesicular bodies with the cellular plasma membrane that deliver their cargo in the form of extracellular vesicles. Containing numerous nonrandomly packed functional proteins, lipids, and RNAs, exosomes are vital intercellular messengers that contribute to the physiologic processes of the healthy organism. During the post‐genome era, exosome‐oriented proteomics have garnered great interest. Since its establishment, mass spectrometry (MS) has been indispensable for the field of proteomics research and has advanced rapidly to interrogate biological samples at a higher resolution and sensitivity. Driven by new methodologies and more advanced instrumentation, MS‐based approaches have revolutionized our understanding of protein biology. As the access to online proteomics database platforms has blossomed, experimental data processing occurs with more speed and accuracy. Here, we review recent advances in the technological progress of MS‐based proteomics and several new detection strategies for MS‐based proteomics research. We also summarize the use of integrated online databases for proteomics research in the era of big data. © 2020 John Wiley & Sons Ltd. Mass Spec Rev