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Electron‐ion reaction‐based dissociation: A powerful ion activation method for the elucidation of natural product structures
Author(s) -
Chen Xiangfeng,
Wang Ze,
Wong Y.L. Elaine,
Wu Ri,
Zhang Feng,
Chan T.W. Dominic
Publication year - 2018
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.21563
Subject(s) - chemistry , dissociation (chemistry) , tandem mass spectrometry , electron transfer dissociation , mass spectrometry , fragmentation (computing) , electron capture dissociation , ion , collision induced dissociation , natural product , combinatorial chemistry , chromatography , stereochemistry , organic chemistry , computer science , operating system
The structural elucidation of natural products (NPs) remains a challenge due to their structurally diversities and unpredictable functionalities, motifs, and scaffolds. Tandem mass spectrometry (MS/MS) is an effective method that assists the full elucidation of complicated NP structures. Ion activation methods play a key role in determining the fragmentation pathways and the structural information obtained from MS/MS. Electron‐ion reaction‐based dissociation (ExD) methods, including electron capture dissociation (ECD), electron transfer dissociation (ETD), electron‐induced dissociation (EID), and electron detachment dissociation (EDD), can induce the breakage of specific chemical bonds and the generation of distinct fragment ions. This review article provides an overview of the mechanisms, instrumentation, and typical applications related to ExD MS/MS in the structural elucidation of NPs, primarly including lipids, oligosaccharides, glycoconjugates, metabolites, and pharmaceutical drugs. This work aims to reveal the capacity and potential of ExD mass spectrometry in analyzing NPs and consequently helping the NP communities to utilize the modern capabilities of MS/MS in the discovery and evaluation of novel NPs.