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Quality control in mass spectrometry‐based proteomics
Author(s) -
Bittremieux Wout,
Tabb David L.,
Impens Francis,
Staes An,
Timmerman Evy,
Martens Lennart,
Laukens Kris
Publication year - 2017
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.21544
Subject(s) - mass spectrometry , chemistry , proteomics , chromatography , workflow , sample (material) , sample preparation , computer science , database , biochemistry , gene
Mass spectrometry is a highly complex analytical technique and mass spectrometry‐based proteomics experiments can be subject to a large variability, which forms an obstacle to obtaining accurate and reproducible results. Therefore, a comprehensive and systematic approach to quality control is an essential requirement to inspire confidence in the generated results. A typical mass spectrometry experiment consists of multiple different phases including the sample preparation, liquid chromatography, mass spectrometry, and bioinformatics stages. We review potential sources of variability that can impact the results of a mass spectrometry experiment occurring in all of these steps, and we discuss how to monitor and remedy the negative influences on the experimental results. Furthermore, we describe how specialized quality control samples of varying sample complexity can be incorporated into the experimental workflow and how they can be used to rigorously assess detailed aspects of the instrument performance.