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Characterization of microbial siderophores by mass spectrometry
Author(s) -
Pluháček Tomáš,
Lemr Karel,
Ghosh Dipankar,
Milde David,
Novák Jiří,
Havlíček Vladimír
Publication year - 2015
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.21461
Subject(s) - siderophore , chemistry , mass spectrometry , inductively coupled plasma mass spectrometry , context (archaeology) , tandem mass spectrometry , chromatography , characterization (materials science) , nanotechnology , biochemistry , paleontology , materials science , biology , gene
Siderophores play important roles in microbial iron piracy, and are applied as infectious disease biomarkers and novel pharmaceutical drugs. Inductively coupled plasma and molecular mass spectrometry (ICP‐MS) combined with high resolution separations allow characterization of siderophores in complex samples taking advantages of mass defect data filtering, tandem mass spectrometry, and iron‐containing compound quantitation. The enrichment approaches used in siderophore analysis and current ICP‐MS technologies are reviewed. The recent tools for fast dereplication of secondary metabolites and their databases are reported. This review on siderophores is concluded with their recent medical, biochemical, geochemical, and agricultural applications in mass spectrometry context. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 35: 35–47, 2016.