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Probing nanoparticles and nanoparticle‐conjugated biomolecules using time‐of‐flight secondary ion mass spectrometry
Author(s) -
Kim YoungPil,
Shon Hyun Kyong,
Shin Seung Koo,
Lee Tae Geol
Publication year - 2014
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.21437
Subject(s) - biomolecule , nanoparticle , chemistry , nanomedicine , secondary ion mass spectrometry , nanotechnology , conjugated system , mass spectrometry , surface modification , chromatography , materials science , polymer , organic chemistry , biochemistry
Bio‐conjugated nanoparticles have emerged as novel molecular probes in nano‐biotechnology and nanomedicine and chemical analyses of their surfaces have become challenges. The time‐of‐flight (TOF) secondary ion mass spectrometry (SIMS) has been one of the most powerful surface characterization techniques for both nanoparticles and biomolecules. When combined with various nanoparticle‐based signal enhancing strategies, TOF‐SIMS can probe the functionalization of nanoparticles as well as their locations and interactions in biological systems. Especially, nanoparticle‐based SIMS is an attractive approach for label‐free drug screening because signal‐enhancing nanoparticles can be designed to directly measure the enzyme activity. The chemical‐specific imaging analysis using SIMS is also well suited to screen nanoparticles and nanoparticle–biomolecule conjugates in complex environments. This review presents some recent applications of nanoparticle‐based TOF‐SIMS to the chemical analysis of complex biological systems. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 34: 237–247, 2015.