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Quantitative mass spectrometric analysis of glycoproteins combined with enrichment methods
Author(s) -
Ahn Yeong Hee,
Kim Jin Young,
Yoo Jong Shin
Publication year - 2014
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.21428
Subject(s) - chemistry , glycosylation , glycoprotein , mass spectrometry , glycoproteomics , chromatography , selected reaction monitoring , lectin , quantitative analysis (chemistry) , proteomics , computational biology , tandem mass spectrometry , biochemistry , glycan , biology , gene
Mass spectrometry (MS) has been a core technology for high sensitive and high‐throughput analysis of the enriched glycoproteome in aspects of quantitative assays as well as qualitative profiling of glycoproteins. Because it has been widely recognized that aberrant glycosylation in a glycoprotein may involve in progression of a certain disease, the development of efficient analysis tool for the aberrant glycoproteins is very important for deep understanding about pathological function of the glycoprotein and new biomarker development. This review first describes the protein glycosylation‐targeting enrichment technologies mainly employing solid‐phase extraction methods such as hydrizide‐capturing, lectin‐specific capturing, and affinity separation techniques based on porous graphitized carbon, hydrophilic interaction chromatography, or immobilized boronic acid. Second, MS‐based quantitative analysis strategies coupled with the protein glycosylation‐targeting enrichment technologies, by using a label‐free MS, stable isotope‐labeling, or targeted multiple reaction monitoring (MRM) MS, are summarized with recent published studies. © 2014 The Authors. Mass Spectrometry Reviews Published by Wiley Periodicals, Inc. Rapid Commun. Mass Spec Rev 34:148–165, 2015.