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Characterization of proteins by ambient mass spectrometry
Author(s) -
Yao ZhongPing
Publication year - 2011
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.20346
Subject(s) - chemistry , mass spectrometry , characterization (materials science) , sample preparation , protein mass spectrometry , sample preparation in mass spectrometry , desorption electrospray ionization , chromatography , electrospray ionization , analytical chemistry (journal) , extractive electrospray ionization , ambient ionization , matrix assisted laser desorption/ionization , ionization , nanotechnology , desorption , chemical ionization , ion , organic chemistry , adsorption , materials science
Proteins play important roles in living systems and are topics of many fundamental and applied research projects. With the introduction of electrospray ionization and matrix‐assisted laser desorption/ionization for analysis of biomacromolecules in the late 1980s, mass spectrometry has become an important tool for characterization of proteins. Characterization of proteins in raw samples by these mass spectrometric techniques, however, usually requires extensive sample pretreatment. Ambient ionization techniques are new mass spectrometric techniques that allow direct analysis of samples with no or little sample preparation. Can these techniques facilitate or even eliminate sample preparation for mass spectrometric analysis of proteins? Apart from sample preparation, do these techniques offer any new features for characterization of proteins as compared with conventional ESI or MALDI? Recent advances in characterization of proteins by ambient mass spectrometry are summarized and commented in this article. © 2011 Wiley Periodicals, Inc. Mass Spec Rev 31:437–447, 2012