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Metabolomic applications of HILIC–LC–MS
Author(s) -
Cubbon Simon,
Antonio Carla,
Wilson Julie,
ThomasOates Jane
Publication year - 2009
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.20252
Subject(s) - hydrophilic interaction chromatography , chemistry , metabolomics , chromatography , mass spectrometry , liquid chromatography–mass spectrometry , chemical polarity , high performance liquid chromatography , organic chemistry , molecule
Hydrophilic interaction liquid chromatography (HILIC), although not a new technique, has enjoyed a recent renaissance with the introduction of robust and reproducible stationary phases. It is consequently finding application in metabolomics studies, which have traditionally relied on the stability of reversed phases (RPs), since the biofluids analyzed are predominantly aqueous and thus contain many polar analytes. HILIC's retention of those polar compounds and use of solvents readily compatible with mass spectrometry have seen its increasing adoption in studies of complex aqueous metabolomes. This review describes the stationary phases and their features, surveys HILIC–LC–MS's role in metabolomics experiments, discusses approaches to data extraction and analysis including multivariate analysis, and reviews the literature on HILIC–MS applications in metabolomics. © 2009 Wiley Periodicals, Inc., Mass Spec Rev 29:671–684, 2010