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Structural characterization of flavonoid glycosides by multi‐stage mass spectrometry
Author(s) -
Vukics Viktoria,
Guttman András
Publication year - 2008
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.20212
Subject(s) - chemistry , flavonoid , glycoside , phytochemical , mass spectrometry , nuclear magnetic resonance spectroscopy , chromatography , organic chemistry , biochemistry , antioxidant
Flavonoids are secondary plant metabolites of great structural variety and high medicinal significance. The search for new chemical entities and the quality control of flavonoid containing natural products require easy‐to‐use but reliable and robust analytical methodologies. For structural elucidation of flavonoids and their glycosides, nuclear magnetic resonance (NMR) and mass spectroscopy (MS) are the generally used techniques. In phytochemical analyses, however, high amounts of flavonoids are difficult to isolate for NMR, thus low sample volume requiring MS based methods are emerging. This review summarizes and compares currently available methods for structural elucidation of flavonoids by LC–MS and LC–MS n , and focuses on the identification options of unknown flavonoid glycosides in complex samples (e.g., plant extracts) with the emphasis on the differentiation of isomeric compounds. © 2008 Wiley Periodicals, Inc., Mass Spec Rev 29:1–16, 2010