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Advances in analysis of microbial metabolic fluxes via 13 C isotopic labeling
Author(s) -
Tang Yinjie J.,
Martin Hector Garcia,
Myers Samuel,
Rodriguez Sarah,
Baidoo Edward E.K.,
Keasling Jay D.
Publication year - 2008
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.20191
Subject(s) - metabolic flux analysis , isotopomers , chemistry , flux (metallurgy) , isotopic labeling , mass spectrometry , metabolomics , tracer , metabolic pathway , isotope , stable isotope ratio , chromatography , analytical chemistry (journal) , metabolism , biochemistry , organic chemistry , physics , nuclear physics , quantum mechanics , molecule
Metabolic flux analysis via 13 C labeling ( 13 C MFA) quantitatively tracks metabolic pathway activity and determines overall enzymatic function in cells. Three core techniques are necessary for 13 C MFA: (1) a steady state cell culture in a defined medium with labeled‐carbon substrates; (2) precise measurements of the labeling pattern of targeted metabolites; and (3) evaluation of the data sets obtained from mass spectrometry measurements with a computer model to calculate the metabolic fluxes. In this review, we summarize recent advances in the 13 C‐flux analysis technologies, including mini‐bioreactor usage for tracer experiments, isotopomer analysis of metabolites via high resolution mass spectrometry (such as GC‐MS, LC‐MS, or FT‐ICR), high performance and large‐scale isotopomer modeling programs for flux analysis, and the integration of fluxomics with other functional genomics studies. It will be shown that there is a significant value for 13 C‐based metabolic flux analysis in many biological research fields. © 2009 Wiley Periodicals, Inc., Mass Spec Rev 28:362–375, 2009