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Proteolytic 18 O‐labeling strategies for quantitative proteomics
Author(s) -
Miyagi Masaru,
Rao K.C. Sekhar
Publication year - 2006
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.20116
Subject(s) - chemistry , proteomics , quantitative proteomics , isobaric labeling , proteolytic enzymes , mass spectrometry , protease , computational biology , bottom up proteomics , protein expression , posttranslational modification , chromatography , biochemistry , tandem mass spectrometry , enzyme , protein mass spectrometry , biology , gene
A number of proteomic techniques have been developed to quantify proteins in biological systems. This review focuses on the quantitative proteomic technique known as “proteolytic 18 O‐labeling.” This technique utilizes a protease and H 2 18 O to produce labeled peptides, with subsequent chromatographic and mass spectrometric analysis to identify and quantify (relative) the proteins from which the peptides originated. The technique determines the ratio of individual protein's expression level between two samples relative to each other, and can be used to quantitatively examine protein expression (comparative proteomics) and post‐translational modifications, and to study protein–protein interactions. The present review discusses various aspects of the 18 O‐labeling technique, including: its history, the advantages and disadvantages of the proteolytic 18 O‐labeling technique compared to other techniques, enzymatic considerations, the problem of variable incorporation of 18 O atoms into peptides with a discussion on recent advancements of the technique to overcome it, computational tools to interpret the data, and a review of the biological applications. © 2006 Wiley Periodicals, Inc., Mass Spec Rev 26:121–136, 2007