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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 1999–2000
Author(s) -
Harvey David J.
Publication year - 2006
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.20080
Subject(s) - chemistry , mass spectrometry , glycoconjugate , chromatography , matrix assisted laser desorption/ionization , surface enhanced laser desorption/ionization , fast atom bombardment , desorption , glycan , protein mass spectrometry , biochemistry , glycoprotein , electrospray ionization , organic chemistry , adsorption
This review describes the use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates and continues coverage of the field from the previous review published in 1999 (D. J. Harvey, Matrix‐assisted laser desorption/ionization mass spectrometry of carbohydrates, 1999, Mass Spectrom Rev, 18:349–451) for the period 1999–2000. As MALDI mass spectrometry is acquiring the status of a mature technique in this field, there has been a greater emphasis on applications rather than to method development as opposed to the previous review. The present review covers applications to plant‐derived carbohydrates, N‐ and O‐ linked glycans from glycoproteins, glycated proteins, mucins, glycosaminoglycans, bacterial glycolipids, glycosphingolipids, glycoglycerolipids and related compounds, and glycosides. Applications of MALDI mass spectrometry to the study of enzymes acting on carbohydrates (glycosyltransferases and glycosidases) and to the synthesis of carbohydrates, are also covered. © 2006 Wiley Periodicals, Inc., Mass Spec Rev 25:595–662, 2006