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Deciphering the human nucleolar proteome
Author(s) -
Couté Yohann,
Burgess Jennifer A.,
Diaz JeanJacques,
Chichester Christine,
Lisacek Frédérique,
Greco Anna,
Sanchez JeanCharles
Publication year - 2005
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.20067
Subject(s) - nucleolus , ribosome biogenesis , biogenesis , chemistry , proteome , ribonucleoprotein , ribosome , proteomics , ribosomal protein , computational biology , human proteome project , stable isotope labeling by amino acids in cell culture , microbiology and biotechnology , interactome , rna , biochemistry , biology , cytoplasm , gene
Nucleoli are plurifunctional nuclear domains involved in the regulation of several major cellular processes such as ribosome biogenesis, the biogenesis of non‐ribosomal ribonucleoprotein complexes, cell cycle, and cellular aging. Until recently, the protein content of nucleoli was poorly described. Several proteomic analyses have been undertaken to discover the molecular bases of the biological roles fulfilled by nucleoli. These studies have led to the identification of more than 700 proteins. Extensive bibliographic and bioinformatic analyses allowed the classification of the identified proteins into functional groups and suggested potential functions of 150 human proteins previously uncharacterized. The combination of improvements in mass spectrometry technologies, the characterization of protein complexes, and data mining will assist in furthering our understanding of the role of nucleoli in different physiological and pathological cell states. © 2005 Wiley Periodicals, Inc. Mass Spec Rev 25:215–234, 2006