Liquid chromatography‐electron ionization mass spectrometry: Fields of application and evaluation of the performance of a Direct‐EI interface

A comprehensive evaluation and a thorough discussion of the fields of possible applications of the Direct‐EI interface are described in this review. Direct‐EI allows the direct introduction of the effluent from a capillary HPLC column into the electron ionization (EI) ion source of a mass spectrometer. Thanks to the reduced liquid intake and an in‐source nebulizer, the interfacing process occurs smoothly and entirely into the ion source. No intermediate interfacing mechanisms of any sort are interposed between the column and the mass spectrometer, thus circumventing any undesired sample loss and minimizing the effort for instrument modification. Theoretically, any GC‐MS system can be converted into an LC‐MS for EI amenable compounds. Several parameters, crucial for a successful integration of liquid chromatography and mass spectrometry, have been considered in the evaluation of the functioning of such an interface: limit of detection, linearity of response, reproducibility, and chromatographic compatibility. Different mobile phases, also containing non‐volatile buffers, were taken into account, demonstrating an outstanding separation flexibility. The entire set of experiments was carried out at different flow rates and temperatures of the ion source. The interface behavior was also tested in real world applications, with mixtures of pesticides, hormones, nitro‐PAH, and endocrine‐disrupting compounds, allowing picogram level detection and the possibility to record library‐matchable, readily interpretable electron ionization mass spectra, for prompt compound characterization and confirmation. © 2005 Wiley Periodicals, Inc., Mass Spec Rev 24:978–989, 2005