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Capillary electrophoresis–mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery
Author(s) -
Kolch Walter,
Neusüß Christian,
Pelzing Matthias,
Mischak Harald
Publication year - 2005
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.20051
Subject(s) - biomarker discovery , chemistry , mass spectrometry , proteome , proteomics , capillary electrophoresis , chromatography , biomarker , capillary electrophoresis–mass spectrometry , biological fluids , computational biology , tandem mass spectrometry , biochemistry , electrospray ionization , biology , gene
Proteome analysis is now emerging as key technology for deciphering biological processes and the discovery of biomarkers for diseases from tissues and body fluids. The complexity and wide dynamic range of protein expression poses a formidable challenge to both peptide separation technologies and mass spectrometry (MS). Here we review the efforts that have been undertaken to date, focussing on capillary electrophoresis coupled to mass spectrometry (CE‐MS). We discuss CE‐MS from an application point of view evaluating its merits and vices in regard to biomarker discovery and clinical applications. As examples, we present the use of CE‐MS for the determination of protein patterns in urine, serum, and other body fluids. Finally, the benefits and limitations of CE‐MS for the analysis of proteins in clinical samples are discussed against the background of alternative technologies. © 2005 Wiley Periodicals, Inc., Mass Spec Rev 24:959–977, 2005