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Application of secondary ion and matrix‐assisted laser desorption‐ionization time‐of‐flight mass spectrometry for the quantitative analysis of biological molecules
Author(s) -
Muddiman David C.,
Gusev Arkady I.,
Hercules David M.
Publication year - 1995
Publication title -
mass spectrometry reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 126
eISSN - 1098-2787
pISSN - 0277-7037
DOI - 10.1002/mas.1280140603
Subject(s) - chemistry , mass spectrometry , sample preparation , ionization , surface enhanced laser desorption/ionization , analytical chemistry (journal) , quantitative analysis (chemistry) , matrix assisted laser desorption/ionization , matrix (chemical analysis) , desorption , maldi imaging , secondary ion mass spectrometry , chromatography , ion , sample preparation in mass spectrometry , electrospray ionization , organic chemistry , adsorption
This review primarily focuses on the current status of two solid‐state mass spectrometric (MS) techniques, namely, static secondary‐ion (SIMS) and matrix‐assisted laser desorption‐ionization (MALDI) time‐of‐fight (TOF) maSs spectrometry, as applied to the quantification of biological materials. Although a number of articles have appeared in the literature for both SIMS and MALDI quantitative analysis, neither field has been extensively researched nor currently used in general practice. However, several results are very exciting and offer advantages over conventional methods. The abilities and limitations of MALDI and SIMS for quantitative analysis of biological molecules will be discussed at the fundamental and applied level. Use of an internal standard approach, development of instrumental protocols, and sample preparation will be examined in detail. Various examples demonstrating the feasibility of quantitative analysis by MALDI and SIMS, including quantification of in vivo and in vitro metabolism of drugs in real biological matrices, will be reviewed. © 1996 John Wiley & Sons, Inc.