Premium
Photoresponsive Vesicles Enabling Sequential Release of Nitric Oxide (NO) and Gentamicin for Efficient Biofilm Eradication
Author(s) -
Duan Yutian,
Zhang Mingyang,
Shen Zhiqiang,
Zhang Mengdan,
Zheng Bin,
Cheng Sheng,
Hu Jinming
Publication year - 2021
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.202000759
Subject(s) - biofilm , vesicle , amphiphile , ethylene oxide , aqueous solution , pseudomonas aeruginosa , chemistry , bacteria , atom transfer radical polymerization , copolymer , nitric oxide , biophysics , combinatorial chemistry , microbiology and biotechnology , biochemistry , organic chemistry , biology , membrane , polymer , genetics
The development of new antibacterial agents that can efficiently eradicate biofilms is of crucial importance to combat persistent and chronic bacterial infections. Herein, the fabrication of photoresponsive vesicles capable of the sequential release of nitric oxide (NO) and gentamicin sulfate (GS) is reported, which can not only efficiently disperse Pseudomonas aeruginosa ( P. aeruginosa ) PAO1 biofilm but also kill the planktonic bacteria. Well‐defined amphiphilic diblockcopolymers of poly(ethylene oxide)‐ b ‐poly(4‐((2‐nitrobenzyl)(nitroso)amino)benzyl methacrylate) (PNO) is first synthesized through atom transfer radical polymerization (ATRP). The PNO diblock copolymer self‐assembled into vesicles in aqueous solution, and a hydrophilic antibiotic of GS is subsequently encapsulated into the aqueous lumens of vesicles. The vesicles undergo visible light‐mediated N‐NO cleavage, releasing NO and disintegrating the vesicles with the release of the GS payload. The sequential release of NO and GS efficiently eradicate P. aeruginosa PAO1 biofilm and kill the liberated bacteria, showing a better antibiofilm effect than that of NO or GS alone.