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Hydrogels with Cell Adhesion Peptide‐Decorated Channel Walls for Cell Guidance
Author(s) -
Wang Shuang,
Sarwat Mariah,
Wang Peng,
Surrao Denver C.,
Harkin Damien G.,
St John James A.,
Bolle Eleonore C. L.,
Forget Aurelien,
Dalton Paul D.,
Dargaville Tim R.
Publication year - 2020
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.202000295
Subject(s) - self healing hydrogels , ethylene glycol , peptide , cell adhesion , adhesion , biophysics , maleimide , materials science , surface modification , nanotechnology , chemistry , polymer chemistry , biochemistry , composite material , biology , organic chemistry
A method is reported for making hollow channels within hydrogels decorated with cell‐adhesion peptides exclusively at the channel surface. Sacrificial fibers of different diameters are used to introduce channels within poly(ethylene glycol) hydrogels crosslinked with maleimide‐thiol chemistry, which are backfilled with a cysteine‐containing peptide solution which is conjugated to the lumen with good spatial efficiency. This allows for peptide patterning in only the areas of the hydrogel where they are needed when used as cell‐guides, reducing the amount of required peptide 20‐fold when compared to bulk functionalization. The power of this approach is highlighted by successfully using these patterned hydrogels without active perfusion to guide fibroblasts and olfactory ensheathing cells—the latter having unique potential in neural repair therapies.