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Biomolecule Sensor Based on Azlactone‐Modified Hydrogel Films
Author(s) -
Li Jie,
Yu Xiaoqian,
Herberg Artjom,
Kuckling Dirk
Publication year - 2019
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201800674
Subject(s) - biomolecule , streptavidin , materials science , surface plasmon resonance , polymer , copolymer , polymerization , spin coating , chain transfer , chemical engineering , polymer chemistry , nanotechnology , coating , chemistry , radical polymerization , biotin , nanoparticle , composite material , biochemistry , engineering
A 3D hydrogel layer is probed by combining surface plasmon resonance with optical waveguide spectroscopy to detect biomolecules. A template terpolymer P(DMAAm‐ co ‐DMIAAm‐ co ‐VDMA) is synthesized via reversible addition‐fragmentation chain‐transfer polymerization. The terpolymer is then modified with an amino group bearing biotin to enable biomolecular recognition for streptavidin. A hydrogel thin layer is prepared onto a gold surface after spin‐coating and photo‐crosslinking of the modified polymer. Finally, the hydrogel is utilized to quantitatively detect streptavidin by using surface plasmon resonance–optical waveguide spectroscopy measurements.