Premium
DNA Block Macromolecules Based on Rolling Circle Amplification Act as Scaffolds to Build Large‐Scale Origami Nanostructures
Author(s) -
Zhang Zhiqing,
Zhang Hongzhi,
Wang Fang,
Zhang Guodong,
Zhou Ting,
Wang Xiufeng,
Liu Shuzhen,
Liu Tingting
Publication year - 2018
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201800263
Subject(s) - rolling circle replication , dna origami , nanostructure , dna , block (permutation group theory) , nanotechnology , base pair , macromolecule , scale (ratio) , pairing , dna nanotechnology , materials science , nanoscopic scale , chemistry , physics , geometry , mathematics , dna replication , biochemistry , superconductivity , quantum mechanics
A simplified origami strategy to create large‐scale complex DNA nanostructures based on the rolling circle amplification (RCA) is developed. The long repetitive block single strand DNA (ssDNA) synthesized from RCA with a few staple strands are used to fabricate DNA origami, which can be assembled into the first level assemblies of micro‐scale length mono‐nanoladders. Depending on base pairing among the sticky ends of short staple strands in nanoladders, the second level assemblies with large‐scale and controlled structures such as tri‐nanoladder, penta‐nanoladder, and even nanobrocade, can be further achieved.