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Hyaluronic Acid–Modified Porous Silicon Films for the Electrochemical Sensing of Bacterial Hyaluronidase
Author(s) -
Tücking KatrinStephanie,
Vasani Roshan B.,
Cavallaro Alex A.,
Voelcker Nicolas H.,
Schönherr Holger,
PrietoSimon Beatriz
Publication year - 2018
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201800178
Subject(s) - hyaluronidase , porous silicon , ferricyanide , hyaluronic acid , differential pulse voltammetry , self healing hydrogels , methacrylate , electrode , electrochemistry , polyethylene glycol , chemistry , cyclic voltammetry , nuclear chemistry , redox , chromatography , materials science , silicon , polymer chemistry , polymer , inorganic chemistry , organic chemistry , enzyme , polymerization , biology , genetics
The development of enzyme‐responsive hyaluronic acid methacrylate (HYAMA)‐coated porous silicon (pSi) films and their application in electrochemical diagnostic devices for the in situ detection of the enzyme hyaluronidase (hyal), which is secreted by Staphylococcus aureus (S. aureus) bacteria, are reported. The approach relies on a HYAMA‐pSi electrode made of thermally hydrocarbonized pSi (pSi‐THC) that is impregnated with crosslinked HYAMA/polyethylene glycol diacrylate (PEGDA) hydrogels. The enzymatic degradation of HYAMA by bacterial hyal is monitored by differential pulse voltammetry (DPV) utilizing pSi‐THC as a working electrode and ferro/ferricyanide (FF) as external redox probe. The degradation of HYAMA results in reduced diffusion of the redox probe through the partially charged film, thereby enabling the detection of hyal by DPV. In addition to the determination of the concentration‐dependent response in NaOAc buffer (pH 5.2), the detection of hyal as indicator for the presence of S. aureus bacteria above a threshold level in bacterial supernatants and artificial wound fluid is highlighted.