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A Novel Water‐Soluble Fluorescence Probe with Wash‐Free Cellular Imaging Capacity Based on AIE Characteristics
Author(s) -
Qian Yunxia,
Liu Hongmei,
Tan Haijian,
Yang Qingmin,
Zhang Shuchen,
Han Lingui,
Yi Xuegang,
Huo Li,
Zhao Hongchi,
Wu Yonggang,
Bai Libin,
Ba Xinwu
Publication year - 2017
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201600684
Subject(s) - fluorescence , polyelectrolyte , fluorescence lifetime imaging microscopy , polymer , polymerization , chemistry , confocal , gel permeation chromatography , polymer chemistry , biophysics , materials science , organic chemistry , physics , biology , geometry , mathematics , quantum mechanics
A potential real‐time imaging water‐soluble fluorescent polymer ( P3 ) is facilely prepared via one‐pot method. For P3 , tetraphenylethene unit serves as the fluorescent unit, poly(acryloyl ethylene diamine) (a kind of polyelectrolyte) with specific degree of polymerization acts as water‐soluble part. 1 H‐NMR, gel permeation chromatography (GPC), UV‐vis spectroscopy, photoluminescence (PL), and confocal laser scanning microscopy are undertaken to characterize the structure and property of P3 . The results of wash‐free cellular imaging show that the signal‐to‐noise ratio is high as the concentration of P3 is 50 μg mL −1 . In addition, the pH‐responsive and Cd 2+ ‐responsive are also investigated in this paper. The results coming from pH‐responsive show that P3 solution displays significant fluorescence under near neutral. And the result from the cellular imaging shows that intracellular fluorescence intensity enhances with the augment of concentration of Cd 2+ , which reveals that P3 can give a hint to resolve the dilemma of traditional fluorescent dyes used as living cellular fluorescent probe.