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Layer‐by‐Layer Assembly of Metal–Organic Frameworks in Macroporous Polymer Monolith and Their Use for Enzyme Immobilization
Author(s) -
Wen Liyin,
Gao Aicong,
Cao Yao,
Svec Frantisek,
Tan Tianwei,
Lv Yongqin
Publication year - 2016
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201500705
Subject(s) - monolith , immobilized enzyme , imidazolate , zeolitic imidazolate framework , materials science , chemical engineering , polymer , layer (electronics) , layer by layer , glycidyl methacrylate , methacrylate , imidazole , chemistry , metal organic framework , polymer chemistry , adsorption , monomer , organic chemistry , nanotechnology , enzyme , catalysis , composite material , engineering
New monolithic materials comprising zeolitic imidazolate framework (ZIF‐8) located on the pore surface of poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) monolith previously functionalized with N ‐(3‐aminopropyl)‐imidazole have been prepared via a layer‐by‐layer self‐assembly strategy. These new ZIF‐8@monolith hybrids are used as solid‐phase carriers for enzyme immobilization. Their performance is demonstrated with immobilization of a model proteolytic enzyme trypsin. The best of the conjugates enable very efficient digestion of proteins that can be achieved in mere 43 s.