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Coacervation of Elastin‐Like Recombinamer Microgels
Author(s) -
Singh Smriti,
Demco Dan Eugen,
Rahimi Khosrow,
Fechete Radu,
RodriguezCabello José Carlos,
Möller Martin
Publication year - 2016
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201500457
Subject(s) - coacervate , relaxometry , magic angle spinning , chemistry , miniemulsion , nuclear magnetic resonance spectroscopy , chemical engineering , dynamic light scattering , materials science , polymer chemistry , polymer , chromatography , nanotechnology , nanoparticle , organic chemistry , spin echo , medicine , monomer , magnetic resonance imaging , engineering , radiology
The investigation of the coacervation (self‐aggregation) behavior of biomicrogels which can potentially be used as drug carriers is an important topic, because self‐aggregation can not only cause loss of activity, but also toxicity and immunogenicity. To study this effect microgels from elastin‐like recombinamer are synthesized using miniemulsion technique. The existence of coacervation for such microgels, at different concentrations and temperatures, is studied and proved by cryo‐field emission scanning clectron microscopy (cryo‐FESEM), cryo‐transmission electron microscopy (cryo‐TEM), and by a novel 1 H high‐resolution magic angle sample spinning (HRMAS), nuclear magnetic resonance (NMR) spectroscopy, and relaxometry methods. The findings by 1 H HRMAS NMR spectroscopy and relaxometry show simultaneous processes of volume phase temperature transition and coacervation with different sensitivity for hydrophobic and hydrophilic amino acid side‐chains in the microgel. The coacervation process is more evidential by the behavior of glycine α‐CH 2 , 1 H NMR peak as compared to the proline β ‐ CH 2 .