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The Guanidinium Group as a Key Part of Water‐Soluble Polymer Carriers for siRNA Complexation and Protection against Degradation
Author(s) -
Tabujew Ilja,
Freidel Christoph,
Krieg Bettina,
Helm Mark,
Koynov Kaloian,
Müllen Klaus,
Peneva Kalina
Publication year - 2014
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201400120
Subject(s) - methacrylamide , copolymer , microscale thermophoresis , polymer , agarose , chemistry , polymer chemistry , chromatography , organic chemistry , biochemistry , acrylamide
Here, the preparation of a novel block copolymer consisting of a statistical copolymer N‐(2‐hydroxypropyl) methacrylamide‐s‐N‐(3‐aminopropyl) methacrylamide and a short terminal 3‐guanidinopropyl methacrylamide block is reported. This polymer structure forms neutral but water‐soluble nanosized complexes with siRNA. The siRNA block copolymer complexes are first analyzed using agarose gel electrophoresis and their size is determined with fluorescence correlation spectroscopy. The protective properties of the polymer against RNA degradation are investigated by treating the siRNA block copolymer complexes with RNase V1. Heparin competition assays confirm the efficient release of the cargo in vitro. In addition, the utilization of microscale thermophoresis is demonstrated for the determination of the binding strength between a fluorescently labeled polyanion and a polymer molecule.