Rapid Hybridization of Chitosan‐Gold‐Antibodies via Metal‐free Click in Water‐based Systems: A Model Approach for Naked‐eye Detectable Antigen Sensors | Zendy

Jirawutthiwongchai Jatesuda | Zendy; Draeger Gerald | Zendy; Chirachanchai Suwabun | Zendy

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Rapid Hybridization of Chitosan‐Gold‐Antibodies via Metal‐free Click in Water‐based Systems: A Model Approach for Naked‐eye Detectable Antigen Sensors

Author(s) -

Jirawutthiwongchai Jatesuda,

Draeger Gerald,

Chirachanchai Suwabun

Publication year - 2014

Publication title -

macromolecular rapid communications

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.348

H-Index - 154

eISSN - 1521-3927

pISSN - 1022-1336

DOI - 10.1002/marc.201400092

Subject(s) - naked eye , click chemistry , antibody , chitosan , antigen , colloidal gold , chemistry , nanotechnology , materials science , combinatorial chemistry , biology , biochemistry , immunology , chromatography , nanoparticle , detection limit

A surface plasmon resonance (SPR) expression after hybridization of chitosan–gold nanoparticle‐antibody ( CS ‐ AuNPs‐Ab ) based on: i) metal‐free click chemistry, and, ii) in water system as an approach for a rapid antigen sensing, is proposed. The chitosan‐hydroxybenzyl triazole complex enables us to carry out the conjugation of mPEG and trifluoromethylated oxanorbornadiene ( OND ) in water. CS‐mPEG‐OND further allows metal‐free click to hybridize chitosan ( CS ) with azido‐modified gold nanoparticles ( azido‐AuNPs ) in aqueous solution at room temperature. The CS‐mPEG‐OND conjugated with LipL32 antibody ( Ab ) not only effectively binds with LipL32 antigen ( Ag ) but also performs the cycloaddition with azido‐AuNPs to display a change in color within 2 min. The phenomenon leads to a simple and efficient naked‐eye antigen detection technique.

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